A mother and two children are the three Irish citizens or dependents who have been safely evacuated from Iran after the US joined Israel's bombing campaign on the country.
It comes after the successful evacuation of 15 Irish citizens and their dependents from Israel yesterday.
The Taoiseach said today Ireland would have preferred if the US had engaged in dialogue with Iran on the "nuclear issue."
"It's our view that those efforts should have been exhausted with Iran and the international community.
"What is required now is a complete de-escalation, an ending of the war.
"And also, we cannot forget Gaza.
"There's a huge danger that Gaza would be sidelined and marginalised in terms of the international profile.
"The continuing loss of life in Gaza is appalling," he said.
The Taoiseach said Iran had been a malign actor in the Middle East for quite some time.
"It even got involved in the Ukraine war by providing drones and so forth, but in particular, its support of Hamas, Hezbollah, the Houthis, its position of destroying Israel, openly declared, makes for a very complex scenario in the Middle East."
Tánaiste and Minister for Foreign Affairs Simon Harris said those who had left Iran will now "travel home".
"I want to extend our gratitude to our European friends and partners for their excellent support."
He added: "Throughout this crisis, the Department of Foreign Affairs and Trade and our Embassies have remained in regular contact with Irish citizens in the region."
Those who have been evacuated were among 29 Irish citizens in Iran who had registered with the Irish embassy and around 200 in Israel.
It comes after the US joined bombing raids on Iran, with Mr Harris saying the US move was an "extraordinarily dangerous escalation" in an already "volatile" situation in the Middle East.
He is attending a meeting of EU foreign affairs ministers in Brussels, where the growing crisis in the Middle East will be discussed among other topics.
Tensions in the Middle East boiled over ten days ago when Israel launched strikes on Tehran, targeting the country's nuclear programme.
Israel and Iran have been exchanging strikes with one another since, rekindling fears of a full-scale conflict.
On Saturday the US weighed in, sending planes and submarines to attack Fordow, Isfahan and Natanz, which are linked to Iran's nuclear programme.
Iran threatened to retaliate, while US President Donald Trump warned of further action if necessary.
"Aspects of it, or elements could be suspended with a QMV I understand, but that is still work to be undertaken, but it simply isn't acceptable that the human rights clauses of that agreement would be so clearly broken," he said.
Speaking ahead of today's EU meeting, Mr Harris said there needed to be "a diplomatic solution" to Iran's nuclear programme.
The Tánaiste said that urgent de-escalation and diplomacy are "crucial", and the EU "has a role to play in charting a way forward".
He said he would also be calling for "swift and concrete actions" in response to the EU's review of the EU-Israel Association Agreement.
"We must also maintain a strong focus on the situation in Gaza, which remains catastrophic," he added.
"I call on Israel to lift its blockade on aid into Gaza. The situation in the West Bank also continues to be unacceptable.
"The EU can and must provide leadership on the humanitarian crisis in Gaza, on the release of hostages, on preserving the two-State solution, and on efforts to support peace and stability in the Middle East."
Micheál Martin also said today that the EU-Israel Association Agreement should now be suspended.
"To achieve that would require unanimity, so we certainly would be looking for actions in the context of that agreement.
"Aspects of it, or elements could be suspended with a QMV I understand, but that is still work to be undertaken, but it simply isn't acceptable that the human rights clauses of that agreement would be so clearly broken," he said.
EU ministers are expected to discuss the latest developments, the situation in Gaza and the West Bank, and the review of Israel's compliance with Article 2 of the EU-Israel Association Agreement.
Among the other topics to be discussed are the Russian invasion of Ukraine, EU engagement with China, and Georgia.
Ukrainian foreign minister Andrii Sybiha will also attend the summit and provide an update.
